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Research Projects
Prevention of thrombotic diseases such as myocardial infarction, stroke etc is a big social problem at present. Thrombosis is also involved in diseases such as cognition disease, diabetes mellitus and other various life-related diseases and in long-flight syndrome. To prevent life-related atherothrombotic diseases, intake of suitable diet and regular physical exercise are recommended. We have studied on basic and practical projects with researchers and clinicians of other facilities.
They are as follows.
A. Studies on thrombosis and thrombolysis
Grown vessels in rabbit ear chamber
Experimental helium-neon laser-induced thrombosis in a rat mesenteric arteriole
Experimental helium-neon laser-induced thrombosis in a rat mesenteric venule
Experimental helium-neon laser-induced thrombosis in a mouse carotid artery
Size calculation of a thrombus formed in a mouse carotid artery by image analysis
Lymphatic flow in rat mesentery
‚aDArteriosclerosis in apolipoprotein E and LDL receptor double deficient mouse
‚bDExploration of fruits, vegetable and other foods with antithrombotic activity
‚cDExamination of antioxidant activity and function of foods
‚dDEvaluation of antithrombotic agents and their mechanism
‚eDEffect of physical exercise on thrombotic status
‚fDMeasurement of thrombotic status of patients with thrombotic disorders
In vivo and in vitro thrombosis models are used in our laboratory. Thrombus is formed in animal blood vessels by irradiating vessels with helium-neon laser and mechanism of thrombogenesis/thrombolysis and antithrombotic effect of various materials are investigated. In an in vitro model, platelet-rich or arterial thrombus is initiated by blood flow called shear force and mechanism of thrombogenesis/thrombolysis and antithrombotic effect of various materials are investigated. In addition, non-anticoagulated blood is used as samples. The instruments which we have used are Haemostatometer and recently developed Global Thrombosis Test (GTT), (http://www.globalthrombosis.com/index.htm). Non-anticoagulated blood and shear force are very important key words in our research. Especially, GTT is developed for bed-side used to aim diagnosis and treatment of patients. Thrombotic status (platelet reactivity as well as thrombolytic activity) can be measured in about 30 minutes. But GTT is suitable not only for patients but for research.
Thrombus is formed by the interaction between blood cells, blood vessels and blood flow (Virchowfs triad). Generally, molecular thrombotic markers and simplified assay systems are used to investigate thrombosis/thrombolysis mechanism and thrombotic status. We have investigated on the basis of the data obtained by this approach. But we have employed more physiological approach. Platelet reactivity and thrombolytic activity are measured globally using Haemostatometry and GTT using non-anticoagulated blood and shear force. Endothelial function has been assessed by vasodilation in vivo (hyperemia) in addition of excised artery ring.
Prevention of life-related diseases is a big social problem. As many know, to prevent these diseases, suitable foods and regular physical exercise and relaxation or escape from much stress are required. We have concentrated to find fruits and vegetables with antithrombotic activity and thrombolytic activities. We have published some scientific papers and some of the results are sold on the market. In addition, assessment of thrombotic status, platelet reactivity and thrombolytic activity, has been started in some hospitals using GTT.
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